Rat embryo E18 full length cDNA is made of RNA extracted from whole embryo using classical guanidine isothiocyanate-phenol:chloroform extraction method. RNA is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with oligo dT primer.
Quality Control: The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
The synthesized cDNA is also used as template for PCR amplification of ß-actin gene. PCR product of ß-actin was visualized on 1% agarose gel.
Applications: cDNA is ideal for gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.
Packing/shipping: each cDNA sample is routinely shipped on dry ice in 1.5 ml vials and is enough for 30 reactions (30 PCR amplifications).
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
- Zyagen Laboratories
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◆发表日期：2012-4-30
Zyagen Laboratories
美国Zyagen Laboratories公司位于加利福尼亚州的生物硅谷圣地亚哥，是一家著名的提供基因表达产品和服务的生物公司。从2000年到现在，在基因表达方面的研究及产品的推广一直处于全球领先地位。Zyagen Laboratories公司由一批训练有素的高水平的学者与研究人员，同时与发展中国家的科学家在生物制药，生物技术等方面有着广泛的合作。
Zyagen Laboratories公司开发和生产销售细胞定位，基因表达及其蛋白质产物的定量分析的生命科学研究产品。Zyagen Laboratories也提供组织学和药物分析等与政府和学术机构合作的全球定制研究服务。Zyagen公司正在不断改进和扩大产品和服务线，以满足越来越多的科学家在生物医学、生命科学研究的需求。Zyagen公司的目标是致力提供简单化、完整化，高质量，价格实惠的基因表达产品及服务。
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