Monkey Skeletal Muscles, Rhesus mRNA (Poly A+ RNA) is purified from total RNA extracted from freshly harvested tissues using classical guanidine isothiocyanate–phenol:chloroform extraction method and treated with DNase to remove residual DNA contamination. Each poly A+ RNA sample is enriched for mRNA transcripts with two rounds of oligo(dT)-cellulose purification, precisely quantified, and stored at -80oC.
Quality Control: The integrity of each RNA sample is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
Applications: RNA is ideal for investigation of rare transcript using qPCR or Northern blot analysis, preparation of cDNA for RT-PCR/RACE analyses, and expression standard in microarray applications.
Packing/shipping: Total RNA sample is routinely provided in RNase-free water at a concentration of 0.25mg/ml or 0.5mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer. Samples are shipped on dry ice.
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
Zyagen Laboratories
美国Zyagen Laboratories公司位于加利福尼亚州的生物硅谷圣地亚哥,是一家著名的提供基因表达产品和服务的生物公司。从2000年到现在,在基因表达方面的研究及产品的推广一直处于全球领先地位。Zyagen Laboratories公司由一批训练有素的高水平的学者与研究人员,同时与发展中国家的科学家在生物制药,生物技术等方面有着广泛的合作。
Zyagen Laboratories公司开发和生产销售细胞定位,基因表达及其蛋白质产物的定量分析的生命科学研究产品。Zyagen Laboratories也提供组织学和药物分析等与政府和学术机构合作的全球定制研究服务。Zyagen公司正在不断改进和扩大产品和服务线,以满足越来越多的科学家在生物医学、生命科学研究的需求。Zyagen公司的目标是致力提供简单化、完整化,高质量,价格实惠的基因表达产品及服务。
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