ATG14Antibody:Autophagy,theprocessofbulkdegradationofcellularproteinsthroughanautophagosomic-lysosomalpathwayisimportantfornormalgrowthcontrolandmaybedefectiveintumorcells.Itisinvolvedinthepreservationofcellularnutrientsunderstarvationconditionsaswellasthenormalturnoverofcytosoliccomponents.ThisprocessisnegativelyregulatedbyTOR(Targetofrapamycin)throughphosphorylationofautophagyproteinATG1.ATG14isasubunitofaclassIIIphosphatidylinositol(PtdIns)3-kinasecomplexthattargetsthecomplextotheendoplasmicreticulum,thesiteofautophagosomeformation.
AdditionalNames
ATG14Antibody:Autophagy-related14,ATG14L,beclin1-associatedautophagy-relatedkeyregulator,beclin1-interactingprotein,BARKORStorage
ATG14antibodycanbestoredat4?Cforthreemonthsand-20?C,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.Reference
- GozuacikDandKimchiA.Autophagyasacelldeathandtumorsuppressormechanism.Oncogene2004;23:2891-906.
- KisenGO,TessitoreL,CostelliP,etal.Reducedautophagicactivityinprimaryrathepatocellularcarcinomaandasciteshepatomacells.Carcinogenesis1993;14:2501-5.
- KamadaY,FunakoshiT,ShintaniT,etal.Tor-mediatedinductionofautophagyviaApg1proteinkinasecomplex.J.Cell.Biol.2000;150:1507-13.
- ItakuraE,KishiC,InoueK,etal.Beclin1formstwodistinctphosphatidylinositol3-kinasecomplexeswithmammalianAtg14andUVRAG.Mol.Biol.Cell2008;19:5360-72.]
PositiveControl
- Cat.No.1308-HumanSmallIntestineTissueLysate]
Formulation
PhysicalState:Liquid
Concentration:1mg/mLProteinGINumber
50540545ProteinAccessionNumber
NP_055739MolecularWeight
54kDaDisclaimer
Thisproductisforresearchuseonly.TestedApplication
E,WB,IFBlockingPeptide
Cat.No.ZP-7043-ATG14PeptideUserNote
Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.Conjugate
NonePurification
ATG14Antibodyisaffinitychromatographypurifiedviapeptidecolumn.Clonality/Clone
ThisisapolyclonalATG14Antibody.Isotype
IgGHost
ATG14antibodywasraisedinrabbit.
Pleaseuseanti-rabbitsecondaryantibodies.Specificity
AtleastthreeisoformsofATG14areknowntoexist;thisantibodywilldetectallthreeisoformsImmunogen
ATG14antibodywasraisedagainstan18aminoacidsyntheticpeptidenearthecenterofhumanATG14.Application
ATG14antibodycanbeusedfordetectionofATG14byWesternblotat1-2μg/mL.Forimmunofluorescencestartat20μg/mL.Buffer
ATG14AntibodyissuppliedinPBScontaining0.02%sodiumazide.SpeciesReactivity
H,M,R