ATG13Antibody:Autophagy,theprocessofbulkdegradationofcellularproteinsthroughanautophagosomic-lysosomalpathwayisimportantfornormalgrowthcontrolandmaybedefectiveintumorcells.Itisinvolvedinthepreservationofcellularnutrientsunderstarvationconditionsaswellasthenormalturnoverofcytosoliccomponents.ThisprocessisnegativelyregulatedbyTOR(Targetofrapamycin)throughphosphorylationofautophagyproteinATG1.ATG13formsacomplexwithULK1andULK2,themammalianhomologsofATG1,andwithFIP200.ThiscomplexisatargetofTORphosphorylationundernormalconditions;inhibitionofTORbyrapamycinorleucinedeprivationleadstodephosphorylationofATG13,ULK1andULK2,whichthenleadstoautophagy.KnockdownofATG13inhibitsautophagosomeformation.
Description
WesternblotanalysisofATG13inrathearttissuelysatewithATG13antibodyat1μg/mLin(A)theabsenceand(B)thepresenceofblockingpeptide.
ImmunohistochemistryofATG13inmouseheartwithATG13antibodyat5μg/mL.
AdditionalNames
ATG13Antibody:Autophagy-relatedprotein13Disclaimer
Thisproductisforresearchuseonly.UserNote
Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.Immunogen
ATG13antibodywasraisedagainsta15aminoacidsyntheticpeptidenearthecarboxyterminusofhumanATG13.Isotype
IgGClonality/Clone
ThisisapolyclonalATG13Antibody.Conjugate
NoneFormulation
PhysicalState:Liquid
Concentration:1mg/mLPurification
ATG13Antibodyisaffinitychromatographypurifiedviapeptidecolumn.Host
ATG13antibodywasraisedinrabbit.
Pleaseuseanti-rabbitsecondaryantibodies.Application
ATG13antibodycanbeusedfordetectionofATG13byWesternblotat1μg/mL.Antibodycanalsobeusedforimmunohistochemistrystartingat5μg/mL.Forimmunofluorescencestartat20μg/mL.TestedApplication
E,WB,IHC,IFBuffer
ATG13AntibodyissuppliedinPBScontaining0.02%sodiumazide.BlockingPeptide
Cat.No.ZP-5799-ATG13PeptideStorage
ATG13antibodycanbestoredat4?Cforthreemonthsand-20?C,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.PositiveControl
- Cat.No.1461-RatHeartTissueLysate]
SpeciesReactivity
H,M,RProteinGINumber
218082953ProteinAccessionNumber
NP_001136145Reference
- GozuacikDandKimchiA.Autophagyasacelldeathandtumorsuppressormechanism.Oncogene2004;23:2891-906.
- KisenGO,TessitoreL,CostelliP,etal.Reducedautophagicactivityinprimaryrathepatocellularcarcinomaandasciteshepatomacells.Carcinogenesis1993;14:2501-
- KamadaY,FunakoshiT,ShintaniT,etal.Tor-mediatedinductionofautophagyviaApg1proteinkinasecomplex.J.Cell.Biol.2000;150:1507-13.
- JungCH,JunCB,RoSH,etal.ULK-Atg13-FIP200complexesmediatemTORsignalingtotheautophagymachinery.Mol.Biol.Cell2009;20:1992-2003.]